Daniel Dan Motlhanka
Botswana University of Agriculture and Natural Resources, Botswana
Title: Comparative phytochemical screening and antioxidant activity of Cardiospermum corindum from Botswana
Biography
Biography: Daniel Dan Motlhanka
Abstract
The aim of this study was to compare the phytochemical composition and antioxidant activity of roots and shoots of Cardiospermum corindum collected from two geographically distant regions of Botswana (Tswapong Hills and Kgale Hills). Qualitative phytochemical analysis revealed the presence of alkaloids, reducing sugars, saponins, phytosterols, phenols, flavonoids, and terpenoids. Analysis by thin layer chromatography revealed that both shoots and roots of plant collected from the two respective regions showed no differences in phytochemical constituents. Total phenol and flavonoid contents were quantitatively estimated. Total phenolic content measured by Folin-Ciocalteu method varied from 164.4±2.2 to 364.2±3.1mg/L (GAE) Gallic Acid Equivalents. The order of total phenol contents was [364.2±3.1](Roots from Tswapong Hills) > [356.0±4.5] (Roots from Kgale Hills) > [169.1±2.6](Shoots from Tswapong Hills) > [164.4±2.2mg/lGAE](Shoots from Kgale Hills). The total flavonoid contents as measured by aluminum chloride method varied from 56.7±1.1 to 124.1±1.5mg/L(QE) Quercetin Equivalents. The order of the total flavonoid contents were [124.1±1.5] (Shoots from Kgale Hills) > [118.8±2.6](Shoots from Tswapong Hills) > [63.3±1.6](Roots from Tswapong Hills) > [56.7±1.1mg/l QE](Roots from Kgale Hills). The antioxidant activity as determined by the DPPH radical scavenging assay revealed that, at all tested concentrations, root extracts exhibited greater (≥86%) scavenging potency than shoot extracts (≤83%). A direct correlation between total phenolic content and free radical scavenging activity was revealed. This work has validated the use of this plant as a health-improving tool. However, structural identification of the bioactive constituents should be carried out.